| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126695 |
| Chromosome: | chromosome 5 |
| Location: | 799771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248200 | (1 of 2) PTHR21493//PTHR21493:SF124 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTAAGACGAGATGATGTTGACAGGTTGG |
| Internal bar code: | CCTTCAGCTTATCTCTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 61.1538 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAACTTTCCGCATTCAT |
| Suggested primer 2: | ACGGATCAATGATGGGATGT |