Insertion junction: LMJ.RY0402.126708_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126708
Chromosome:chromosome 7
Location:6279061
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g356850 (1 of 7) PF00582 - Universal stress protein family (Usp) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAGATTGGACACAGTACGACGGGAGTC
Internal bar code:CGGAGCGGTGATTTCTCGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:927
LEAP-Seq percent confirming:86.8996
LEAP-Seq n confirming:1393
LEAP-Seq n nonconfirming:210
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCTTTCTGTTGTTGGT
Suggested primer 2:GCCCTGGACATACAGGAGAA