| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126713 |
| Chromosome: | chromosome 3 |
| Location: | 7927191 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202202 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCACAACTACACCCTCCGCCGTAGG |
| Internal bar code: | GCACACCCAGAGAGGAAAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 95 |
| LEAP-Seq percent confirming: | 99.7006 |
| LEAP-Seq n confirming: | 333 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCGTACGTGTTGTCGTA |
| Suggested primer 2: | TGCTTGATGCAGATTGGAAG |