| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126727 |
| Chromosome: | chromosome 3 |
| Location: | 8705380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204353 | (1 of 1) K07952 - ADP-ribosylation factor related protein 1 (ARFRP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCCTACCCCATCTTCCTCTGCGTGGC |
| Internal bar code: | TCCGCACCGAGGGGGGCGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 92.9006 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAAGCTGATTCGTCACA |
| Suggested primer 2: | ACTCAGGTATTGCCGTGACC |