| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126757 |
| Chromosome: | chromosome 4 |
| Location: | 2475352 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221750 | (1 of 88) IPR011333 - POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGACCTCTGGCCCAGCAAGGATGCGGCG |
| Internal bar code: | CAGTGGGGGTGCAATCGGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 99.5551 |
| LEAP-Seq n confirming: | 895 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCGTAACGCTTTCGTTC |
| Suggested primer 2: | ATAGAACCACCAAACAGGCG |