Insertion junction: LMJ.RY0402.126788_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.126788
Chromosome:chromosome 10
Location:4716467
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g453650 (1 of 1) PTHR32086//PTHR32086:SF0 - FAMILY NOT NAMED // FANCONI ANEMIA GROUP D2 PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGTACTCAACGCCAAGCCCAGAGGAA
Internal bar code:TATGACGTTAGTTAGTCTAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:99.5986
LEAP-Seq n confirming:1737
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCCTAGTCGTGAGCTGC
Suggested primer 2:CGTCCCTCTCTTCCCTCTCT