Insertion junction: LMJ.RY0402.126827_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.126827
Chromosome:chromosome 6
Location:8381579
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g306800 CGL126 protein tyrosine kinase; (1 of 1) K08851 - TP53 regulating kinase and related kinases [EC:2.7.11.1] (TP53RK, PRPK, BUD32) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCACCACCAACCCAAGTCCTCCTTCGT
Internal bar code:CGTCTCCGGACGTGAACGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:465
LEAP-Seq percent confirming:99.3644
LEAP-Seq n confirming:469
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGACTCACAAAGGCTGA
Suggested primer 2:CAACTCTGCACCGATTTCAA