| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126863 |
| Chromosome: | chromosome 12 |
| Location: | 4475047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521650 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTTTGGCGCCGTGCCGGTGTCGGGGCGC |
| Internal bar code: | CCAGGCACATGTCCCGAATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTTCCTCTGTACGCTG |
| Suggested primer 2: | GGGGTAGCCGTTCATGTAGA |