Insertion junction: LMJ.RY0402.126877_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.126877
Chromosome:chromosome 7
Location:2646775
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g330550 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACCGCCTCGCGCCACCCACCAGTGCGT
Internal bar code:AACGGGTTGGCAAGCGGGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:152
LEAP-Seq percent confirming:20.7304
LEAP-Seq n confirming:1493
LEAP-Seq n nonconfirming:5709
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGCCAACAACTGTCCCT
Suggested primer 2:CATCTGCCCCTCTCTATCCA