| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.126887 |
| Chromosome: | chromosome 12 |
| Location: | 7529002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555800 | STK2,STPK2 | Serine/threonine protein kinase; (1 of 1) PTHR24362//PTHR24362:SF286 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTTGGCTGTCCGGGCCCGTGCGTATT |
| Internal bar code: | TGTTCAAATACTGGTAAACCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 98.43 |
| LEAP-Seq n confirming: | 815 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAATACCGCCGCAAGTACC |
| Suggested primer 2: | ACCAAATTGTTTTCCTTGCG |