| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126928 |
| Chromosome: | chromosome 6 |
| Location: | 4147949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278227 | (1 of 4) 2.3.1.12 - Dihydrolipoyllysine-residue acetyltransferase / Transacetylase X | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGGATGAGCACCCCTACGGTGTAATG |
| Internal bar code: | CGTTCACGCTTTCACGAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1174 |
| LEAP-Seq percent confirming: | 99.2312 |
| LEAP-Seq n confirming: | 7744 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGTATGTAGGCATGGAC |
| Suggested primer 2: | ACCTGTATGCGGATCTGGAC |