| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126952 |
| Chromosome: | chromosome 3 |
| Location: | 4837378 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178950 | HEL17 | (1 of 1) PTHR13710//PTHR13710:SF70 - DNA HELICASE RECQ FAMILY MEMBER // BLOOM SYNDROME PROTEIN; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGGCCTCGGTGGCAGTGGCGGTGAGG |
| Internal bar code: | CCCTAAGCTGGTTGGGTGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.0991 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGCCCCTATCCAACCAG |
| Suggested primer 2: | GCTACGTCAGCAGCACTCAG |