| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.126953 |
| Chromosome: | chromosome 11 |
| Location: | 319397 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467565 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTACGGTATCAATTTGTTGTTCTGTTG |
| Internal bar code: | ATTCCCACCACTGGTGTGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 96.786 |
| LEAP-Seq n confirming: | 3975 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCCTTTAGGGCAATCAC |
| Suggested primer 2: | GAAGATGGTGAGGAGGGTGA |