Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.127024 |
Chromosome: | chromosome_8 |
Location: | 1027805 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362050 | FAP49 | Flagellar Associated Protein, membrane-associated | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACATAATGATGTCGCCGCCCACTCATTGC |
Internal bar code: | TCCCTAGTTCGGGTAACACAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 701 |
LEAP-Seq percent confirming: | 99.3043 |
LEAP-Seq n confirming: | 2712 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGCATGCTACGGTATTTGG |
Suggested primer 2: | ATCGTGGTGTTCAGCAGATG |