Insertion junction: LMJ.RY0402.127024_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.127024
Chromosome:chromosome_8
Location:1027805
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g362050 FAP49 Flagellar Associated Protein, membrane-associated antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TACATAATGATGTCGCCGCCCACTCATTGC
Internal bar code:TCCCTAGTTCGGGTAACACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:99.3043
LEAP-Seq n confirming:2712
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCATGCTACGGTATTTGG
Suggested primer 2:ATCGTGGTGTTCAGCAGATG