| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127053 |
| Chromosome: | chromosome 5 |
| Location: | 3441856 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241350 | (1 of 1) 3.6.3.36 - Taurine-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCCCCGCAGCGAGAACCTGGAGTACA |
| Internal bar code: | GGATCACGGAGGGCAAAGGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 856 |
| LEAP-Seq percent confirming: | 99.317 |
| LEAP-Seq n confirming: | 24576 |
| LEAP-Seq n nonconfirming: | 169 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCTGCTTGTCTCAAAA |
| Suggested primer 2: | ACACACACACACACACACGG |