| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127065 |
| Chromosome: | chromosome 17 |
| Location: | 2074314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712050 | (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTGCGCGCGCCCGCGTGGCCATGTGTG |
| Internal bar code: | CACCGTGTCAGTGCTTTTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 66.1111 |
| LEAP-Seq n confirming: | 238 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCAACATACCACACGTC |
| Suggested primer 2: | GAGCACCTTTACATTCGGGA |