Insertion junction: LMJ.RY0402.127068_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.127068
Chromosome:chromosome 3
Location:1634503
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g152900 MPA5 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGATGCAACTCCGGCCACGTAGTCAAG
Internal bar code:TTGCGCCTGTGAAGTCCTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:135
LEAP-Seq percent confirming:98.2888
LEAP-Seq n confirming:919
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTAGATGAAGGCGCTGG
Suggested primer 2:CTGTGGTCGCTTTTTGGTTT