Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.127068 |
Chromosome: | chromosome 3 |
Location: | 1634503 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g152900 | MPA5 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGATGCAACTCCGGCCACGTAGTCAAG |
Internal bar code: | TTGCGCCTGTGAAGTCCTGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 135 |
LEAP-Seq percent confirming: | 98.2888 |
LEAP-Seq n confirming: | 919 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCTAGATGAAGGCGCTGG |
Suggested primer 2: | CTGTGGTCGCTTTTTGGTTT |