| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127082 |
| Chromosome: | chromosome 17 |
| Location: | 2183051 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713051 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCTCCAAACAGATCGCCAACGTCCATC |
| Internal bar code: | TTGGAGACACCAAACGTCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 74.0899 |
| LEAP-Seq n confirming: | 1038 |
| LEAP-Seq n nonconfirming: | 363 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAATAGACCCACCGCTTG |
| Suggested primer 2: | CACAGGATGACAGCGAAGAA |