| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127087 |
| Chromosome: | chromosome 17 |
| Location: | 2792043 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718750 | FAP309 | EF-Hand Containing Flagellar Associated Protein 309; (1 of 1) IPR001763//IPR002048//IPR011992 - Rhodanese-like domain // EF-hand domain // EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCGTGCAGGCAAATTGAGCTGAAGGA |
| Internal bar code: | GCGCCAAAAAATAGTTTTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.5266 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGAGTTCGTGCATCTCG |
| Suggested primer 2: | GCAGTTTGGAACCTCAGCTC |