Insertion junction: LMJ.RY0402.127096_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.127096
Chromosome:chromosome 11
Location:613330
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467612 (1 of 1) PTHR10108//PTHR10108:SF924 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTACGCCCCGACTCTTCCCACCTGCGGC
Internal bar code:AGGCCGTGAGCAGCCCTGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:438
LEAP-Seq percent confirming:99.3625
LEAP-Seq n confirming:1091
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGATGGACGTTTGGAGTTGG
Suggested primer 2:AGTGGGAAACATGCCGTAAG