Insertion junction: LMJ.RY0402.127103_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127103
Chromosome:chromosome 8
Location:1428878
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g364351 FAL12 Protein of unknown function; (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGACATGCGGCAATCACCGGTATTAATC
Internal bar code:AGGTCGCGGTGATGGGGGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:93.5664
LEAP-Seq n confirming:669
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGACCTAACAACAGGGC
Suggested primer 2:CTTGTTTTGCGTTTCCCATT