| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127114 |
| Chromosome: | chromosome_8 |
| Location: | 992105 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g362000 | FAP72 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGGGCGGCCGTGAAGCCCGACCAGCCGC |
| Internal bar code: | TGAAACGTAGAGGCGCAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.3377 |
| LEAP-Seq n confirming: | 600 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGCACCTCCAGTATCAG |
| Suggested primer 2: | GCACCGTCCAAAATTGACTT |