| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127137 |
| Chromosome: | chromosome 9 |
| Location: | 5200334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399289 | (1 of 3) PF00651//PF03110 - BTB/POZ domain (BTB) // SBP domain (SBP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTTGACACGGCGGGACGAGGCGATGGC |
| Internal bar code: | AGACCCGCTCACTCTGGACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 99.9096 |
| LEAP-Seq n confirming: | 3315 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTATGGCAGGAACACTC |
| Suggested primer 2: | ATCCATGTCCTCATCCTTGG |