| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127147 |
| Chromosome: | chromosome 6 |
| Location: | 2881375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272100 | (1 of 1) K15463 - tRNA A64-2'-O-ribosylphosphate transferase [EC:2.4.2.-] (RIT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGGCAACGTGAACACGGACACGTTGC |
| Internal bar code: | TAGGAGGGCCTCAAATTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.7676 |
| LEAP-Seq n confirming: | 15454 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCGAGCTCCAATCACA |
| Suggested primer 2: | CTGATACACGACACCAACGG |