Insertion junction: LMJ.RY0402.127163_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.127163
Chromosome:chromosome 7
Location:3673225
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g337450 GT90F9,GT90-9,CGL75 Glycosyl transferase GT90 family protein 9; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGCAGTGCACATGCCACCCGTGCTCGC
Internal bar code:TCTAGTAGCTATGGTTGGCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:412
LEAP-Seq percent confirming:94.1281
LEAP-Seq n confirming:529
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATCTAGCTCATACCGGA
Suggested primer 2:CGCTTATCCTCCTTGTGCTC