| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127249 |
| Chromosome: | chromosome 10 |
| Location: | 5556156 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459600 | (1 of 1) K14832 - ribosome biogenesis protein MAK21 (MAK21, NOC1, CEBPZ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTGACGCGGTTGCTAACCCTGCTATG |
| Internal bar code: | AATGGAGATTCCAGAACCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGGCAGTATGGAGGTTG |
| Suggested primer 2: | GAGAAGGCCATGCGTGTAAT |