| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127261 |
| Chromosome: | chromosome 7 |
| Location: | 616354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGCGCATGCTCATGGCTGCCTGCGGT |
| Internal bar code: | CATCGTCCAATTCCCCTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 98.3146 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAAGTGTGTGTGTAGCG |
| Suggested primer 2: | CCAGCTCTCCCGTTACACTC |