Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.127269 |
Chromosome: | chromosome 7 |
Location: | 330879 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g314500 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGATGGTGGGCCAGTGCGGGTTCTGG |
Internal bar code: | GTGCGACGAAGGGCGTACCAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 823 |
LEAP-Seq percent confirming: | 96.0968 |
LEAP-Seq n confirming: | 1231 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCAGGAGAGATGGATGGC |
Suggested primer 2: | TACGGTTATGGCGTGTACGA |