| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127341 |
| Chromosome: | chromosome 10 |
| Location: | 3958109 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448550 | (1 of 3) PF14240 - YHYH protein (YHYH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGGTGCGCGCATACCGCTACGCCACCT |
| Internal bar code: | CCGCGCTTCTGACGGCTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 80.0531 |
| LEAP-Seq n confirming: | 2111 |
| LEAP-Seq n nonconfirming: | 526 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCCTACGCACTCATGC |
| Suggested primer 2: | AGTGCGTCAAGCAATGTGAG |