Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.127348 |
Chromosome: | chromosome_17 |
Location: | 3956709 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g728650 | FAP196 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTCAGTGAATCCGGTTTCTGTAGATTGG |
Internal bar code: | GCCCTGAATGTGGGTGGGTGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 320 |
LEAP-Seq percent confirming: | 99.29 |
LEAP-Seq n confirming: | 1818 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
Suggested primer 2: | GTTTGGCTTGGTTTGGTGAT |