| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127353 |
| Chromosome: | chromosome 17 |
| Location: | 764754 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701450 | (1 of 12) PTHR13078:SF22 - MAOC-LIKE DEHYDRATASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTCGCTGCCGTCGGCCGAGTCCTCCAA |
| Internal bar code: | TTCGTCAGAAAGGTTGATCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.2 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGGATGATTGGCTTCAG |
| Suggested primer 2: | TGGGGCACGGTTAGTAAGAC |