| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127366 |
| Chromosome: | chromosome 12 |
| Location: | 7675748 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554250 | LPB1 | (1 of 1) PTHR11952:SF8 - UDP-GLUCOSE PYROPHOSPHORYLASE 3; Low Photochemical Bleaching protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTGTGACACAACAGAGGCAGCGCGTG |
| Internal bar code: | AGGAATCGGTGTGCGAGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 99.799 |
| LEAP-Seq n confirming: | 4469 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGACGACAGCCTCTGCAT |
| Suggested primer 2: | AACATGGCCGACTACCTGAC |