| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127395 |
| Chromosome: | chromosome 7 |
| Location: | 4630452 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344260 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCTAAACTACATTCACAACTGGATGG |
| Internal bar code: | AGCCGATATCACGCCTTATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 951 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGAAGTTTGTGCGAGC |
| Suggested primer 2: | ACTTACACGGGGCAGATGAC |