| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127404 |
| Chromosome: | chromosome 17 |
| Location: | 6080191 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGTGAGTGGCCCAGGGATACATTGTTT |
| Internal bar code: | AAGCCAAGCGTGGCCCAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 99.6569 |
| LEAP-Seq n confirming: | 2033 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAAAATCTGAAGATGGGC |
| Suggested primer 2: | TCCAGGCTGTGTTGCTACTG |