| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.127417 |
| Chromosome: | chromosome 1 |
| Location: | 6624183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047400 | (1 of 88) 3.6.4.12 - DNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGACTAAACCTGGTGTCCGCGGCCGCGC |
| Internal bar code: | AGTCGACTACCCGGAGTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 99.6764 |
| LEAP-Seq n confirming: | 308 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCTCCTCCTCCTCCT |
| Suggested primer 2: | CCACGGTGCATACTGATACG |