| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127424 |
| Chromosome: | chromosome 7 |
| Location: | 4494147 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343433 | OGD4 | (1 of 3) 2.3.1.61 - Dihydrolipoyllysine-residue succinyltransferase / Succinyl-CoA:dihydrolipoate S-succinyltransferase; Dihydrolipoamide succinyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGAACACGCTCATGAAACCCAGCTTGA |
| Internal bar code: | GTGATGACAGGTGCCGGGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 99.2812 |
| LEAP-Seq n confirming: | 1105 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCGTTTTACGTCCGGT |
| Suggested primer 2: | CTCGATGCCCAAAGACTAGC |