| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127435 |
| Chromosome: | chromosome 10 |
| Location: | 1295278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427350 | CYP31,CYP39A2 | Cytochrome P450, CYP213 superfamily; (1 of 1) 1.14.13.79 - Ent-kaurenoic acid oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCGATGAAGCTCATTCATACTGGTGGA |
| Internal bar code: | GCGCCATAGTTAAACACCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.7778 |
| LEAP-Seq n confirming: | 1347 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAACCGACAGTCAAAGA |
| Suggested primer 2: | GCTTCATGCTTCACTCCACA |