| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127447 |
| Chromosome: | chromosome 16 |
| Location: | 4076520 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGGGCTGGACCCCTCGAAACCATGACC |
| Internal bar code: | TTCTCAGTAAGATCGGCTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 99.561 |
| LEAP-Seq n confirming: | 3402 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACGGAGACCAGGTCAGG |
| Suggested primer 2: | GCCAAGGCACAATGATAACC |