Insertion junction: LMJ.RY0402.127451_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.127451
Chromosome:chromosome 17
Location:1053209
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g703750 (1 of 1) PF14222 - Cell morphogenesis N-terminal (MOR2-PAG1_N) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTTTGTGCAATGGGTTTACAACCCCATC
Internal bar code:TGTATGGCGATTCTTGTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:579
LEAP-Seq percent confirming:98.4925
LEAP-Seq n confirming:392
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGGTTGCCATGTCTAGG
Suggested primer 2:CGCAAGGGACTTGCTTAGAC