| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127455 |
| Chromosome: | chromosome 12 |
| Location: | 5592006 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531900 | FLVA,FLVA1 | (1 of 2) 1.5.3.22 - Coenzyme F420H(2) oxidase; Thylakoid flavodiiron protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCATTGGGCTCACGCAGGATGGTGCC |
| Internal bar code: | GACGCGAGGTCGACACATCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 99.3651 |
| LEAP-Seq n confirming: | 626 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCGTAGCCAGAAACAG |
| Suggested primer 2: | AAGCATGAGGTAACGGGATG |