| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127512 |
| Chromosome: | chromosome 2 |
| Location: | 5172406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105500 | AOD1 | Acetylornithine deacetylase; (1 of 1) 3.5.1.16 - Acetylornithine deacetylase / N-acetylornithinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGTACCCCAGTCTAACGATTGTGTGC |
| Internal bar code: | TGCTTTTCAGCAGATTTATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 99.8144 |
| LEAP-Seq n confirming: | 3764 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGACTTGCTCATATCCAT |
| Suggested primer 2: | CCACCAGGTGTACGGCTACT |