| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127527 |
| Chromosome: | chromosome 8 |
| Location: | 4792076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384320 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACATGAGGATACCATGCATGCTATGCA |
| Internal bar code: | TCTGGTGTGGCAGATAACTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 99.0792 |
| LEAP-Seq n confirming: | 538 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACCACACTGGTACCCT |
| Suggested primer 2: | GGCATTGACAGTTGGTTGTG |