| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127528 |
| Chromosome: | chromosome 2 |
| Location: | 7406383 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144600 | PTB9 | Sodium/phosphate symporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGACGCAGACGCTGCACGTGCCTCCT |
| Internal bar code: | CGCTCGCTCCGACCGACGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 93.3029 |
| LEAP-Seq n confirming: | 613 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTCCAAAAGCGGTACAC |
| Suggested primer 2: | ACTCTGTAATCACCACCGGC |