| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127558 |
| Chromosome: | chromosome_13 |
| Location: | 5166113 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g607750 | FAP250,DRC2 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCAGTTGGTCACAAAGTTAGCCTTCGTTA |
| Internal bar code: | CTCCCCTCGCACACAGCGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.6711 |
| LEAP-Seq n confirming: | 2121 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCCTATCTAGCAGTCGC |
| Suggested primer 2: | TTATCGCTCATCCTCATCCC |