Insertion junction: LMJ.RY0402.127578_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.127578
Chromosome:chromosome 3
Location:3573287
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g168200 C1b-135,FAP69 Flagellar Associated Protein 69; (1 of 4) IPR000225//IPR011989//IPR016024 - Armadillo // Armadillo-like helical // Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAATGTCGCCCTGACGGGCATGAGTAGC
Internal bar code:AGCAGCGACTTCTTGTAGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:835
LEAP-Seq percent confirming:99.6835
LEAP-Seq n confirming:945
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGCAAGAGTGGTGCTA
Suggested primer 2:CTTATGGCAGCTACCAAGCC