| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127589 |
| Chromosome: | chromosome 6 |
| Location: | 6499322 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCTAGCTGCGAGTCAGCAGGCGGAAC |
| Internal bar code: | GTGCGCATAACCGCAAAATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 98.7742 |
| LEAP-Seq n confirming: | 2498 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCCTATCTACCCCAGG |
| Suggested primer 2: | ACAGACTGGCAGACATGCAG |