| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127611 |
| Chromosome: | chromosome 1 |
| Location: | 269441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001671 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAACATAACACAGTGAACGCCTCAAACTC |
| Internal bar code: | AATATTTCTGTGGAGTATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 88.7427 |
| LEAP-Seq n confirming: | 607 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGACAGGTGGTCGGGTG |
| Suggested primer 2: | TACCCAATTTAGCAATCGCC |