| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127615 |
| Chromosome: | chromosome 6 |
| Location: | 7462090 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300139 | (1 of 7) IPR010995 - DNA repair Rad51/transcription factor NusA, alpha-helical | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGGTCGGCCAGGCGGTCCGGGAGGT |
| Internal bar code: | CGGCGGGAAGCGGTCGTCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 61.7391 |
| LEAP-Seq n confirming: | 781 |
| LEAP-Seq n nonconfirming: | 484 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGGAAGCTCTCGACTG |
| Suggested primer 2: | GCTATCAACAGGAGCGGAAG |