| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127634 |
| Chromosome: | chromosome 12 |
| Location: | 8153236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551100 | (1 of 1) PTHR36356:SF1 - EXPRESSED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATAAGATTGCTCGGGGTATCGTTCAATG |
| Internal bar code: | CGGGGGCAGCAAGATCGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 93.4783 |
| LEAP-Seq n confirming: | 11438 |
| LEAP-Seq n nonconfirming: | 798 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCGCATACTTGAAGCG |
| Suggested primer 2: | ACATATGTCGCAACACGCAT |