| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127634 |
| Chromosome: | chromosome 12 |
| Location: | 8153237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551100 | (1 of 1) PTHR36356:SF1 - EXPRESSED PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGTTCATCCCACACAACTGCTAGTGG |
| Internal bar code: | CCCTAGGTCGGGACTAACTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.3737 |
| LEAP-Seq n confirming: | 476 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCGCATACTTGAAGCG |
| Suggested primer 2: | ACATATGTCGCAACACGCAT |